WB result of ORAI3 Recombinant Rabbit mAb
Primary antibody: ORAI3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 28 kDa
This blot was developed with high sensitivity substrate
ORAI3 Recombinant Rabbit mAb (S-2486-117)
ORAI3 Recombinant Rabbit mAb (S-2486-117)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ORAI3 |
| Synonyms | Protein orai-3; Transmembrane protein 142C; TMEM142C |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9BRQ5 |
| Clone Number | S-2486-117 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Positive Sample | NIH/3T3, RAW264.7, A20 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
ORAI3 is a four-transmembrane store-operated Ca²⁺ channel protein that, upon association with the endoplasmic-reticulum Ca²⁺ sensor STIM1, forms highly Ca²⁺-selective CRAC channels mediating store-operated Ca²⁺ entry (SOCE) essential for T- and B-cell activation, endothelial and smooth-muscell function, and metabolic reprogramming; beyond SOCE, ORAI3 can heteromerize with ORAI1 to create arachidonic-acid-activated, store-independent channels, is transcriptionally up-regulated by TGF-β to drive the epithelial–mesenchymal transition gene Snai1, and in many cancers its over-expression promotes stemness, migration, chemoresistance and tumour growth via both Ca²⁺-dependent and Ca²⁺-independent signalling, making it a potential prognostic biomarker and therapeutic target.
Picture
Picture
Western Blot
