OGT Mouse mAb

Catalog Number: S0B60189 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl

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Product Details

Product Specification

Host	Mouse
Antigen	OGT
Synonyms	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit (OGT)
Location	Cytoplasm, Nucleus
Accession	O15294
Antibody Type	Mouse mAb
Isotype	IgG2a
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, HEK-293, NIN/3T3, rat brain
Purification	Protein A
Concentration	1.4 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:2000-1:10000	Hu, Ms, Rt
IHC-P	1:250-1:1000	Hu, Ms, Rt

Background

OGT is a widely expressed enzyme in the human body that primarily catalyzes O-GlcNAcylation, a dynamic and reversible post-translational modification. This modification involves the transfer of a single N-acetylglucosamine (GlcNAc) sugar moiety to serine or threonine residues of proteins, thereby participating in the regulation of various critical cellular processes, including signal transduction, gene expression, cell cycle progression, and stress responses. Structurally, the OGT protein contains an N-terminal domain consisting of 14 tetratricopeptide repeat (TPR) motifs and a C-terminal catalytic domain, where the TPR domain is responsible for recognizing and binding substrate proteins, while the catalytic domain transfers the sugar moiety from the UDP-GlcNAc donor to the target protein. Notably, UDP-GlcNAc is the end product of the hexosamine biosynthetic pathway (HBP), thus making OGT activity a direct reflection of the cellular nutritional status. In terms of pathology, OGT dysregulation is closely associated with various diseases: for instance, in cancer, OGT drives tumor initiation and progression by glycosylating multiple oncogene or tumor suppressor gene products; in neurodegenerative diseases, OGT modulates the function of neuro-related proteins such as tau through glycosylation, influencing disease progression; additionally, OGT also plays an important role in metabolic diseases, where alterations in its expression levels and activity are involved in regulating the expression of metabolism-related genes.

Picture

Western Blot

WB result of OGT Mouse mAb
Primary antibody: OGT Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDa

WB result of OGT Mouse mAb
Primary antibody: OGT Mouse mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDa

WB result of OGT Mouse mAb
Primary antibody: OGT Mouse mAb at 1/10000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 116 kDa
Observed MW: 116 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-OGT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.