WB result of OGN Recombinant Rabbit mAb
Primary antibody: OGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40 kDa
OGN Recombinant Rabbit mAb (S-245-327)
OGN Recombinant Rabbit mAb (S-245-327)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | OGN |
| Synonyms | Mimecan; Osteoglycin; Osteoinductive factor (OIF); OIF; SLRR3A |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Extracellular space |
| Accession | P20774 |
| Clone Number | S-245-327 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, HeLa, mouse skeletal muscle, mouse blood vessel, mouse skin, rat skeletal muscle, rat blood vessel, rat skin |
| Predicted Reactivity | Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
OGN (Osteoglycin), also known as Mimecan or SLRR3A, is a member of the small leucine-rich proteoglycan (SLRP) family and a glycosylated protein involved in the development of the extracellular matrix (ECM). It functions as a growth factor that induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2 and may regulate osteoblast differentiation. OGN is expressed in various tissues and is implicated in multiple biological processes, including fibrosis, tumorigenesis, and cardiovascular events. High expression of OGN has been associated with elevated heart left ventricular mass and may play a role in the progression of certain cancers, such as pancreatic cancer, by targeting ID4. Additionally, OGN is detected in blood through mass spectrometry and proximity extension assays, indicating its potential as a biomarker in diseases like pediatric retinoblastoma.
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Western Blot
WB result of OGN Recombinant Rabbit mAb
Primary antibody: OGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse blood vessel lysate 20 µg
Lane 3: mouse skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40, 25 kDa
WB result of OGN Recombinant Rabbit mAb
Primary antibody: OGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat blood vessel lysate 20 µg
Lane 3: rat skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40, 25 kDa
