WB result of Occludin Recombinant Rabbit mAb
Primary antibody: Occludin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Lane 6: HT-29 whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 18-60 kDa
Occludin Recombinant Rabbit mAb (SDT-4588)
Occludin Recombinant Rabbit mAb (SDT-4588)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Occludin |
| Synonyms | OCLN |
| Location | Cell membrane |
| Accession | Q16625 |
| Clone Number | SDT-4588 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Caco-2, HepG2, A431, A549, HT-29, bEnd.3, mouse colon, mouse small intestine, mouse liver, mouse kidney, mouse brain, mouse lung, rat colon, rat liver, rat kidney, rat brain, rat lung |
| Predicted Reactivity | Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:100 | Hu |
Background
Occludin is a approximately 65 kDa four-pass transmembrane protein, identified and named in 1993 as a pivotal component of tight junctions (TJs), primarily localized at the apical lateral membranes of epithelial and endothelial cells; featuring two extracellular loops, one intracellular loop, and a long C-terminal cytoplasmic tail, its extracellular loops mediate "zipper-like" sealing between adjacent cells via homo- or heterophilic interactions, thereby establishing a selective permeability barrier that strictly regulates paracellular transport and maintains cell polarity, while its C-terminus anchors tight junctions to the actin cytoskeleton by binding to cytoplasmic scaffolding proteins such as ZO-1, subsequently participating in the fine-tuned regulation of cell signaling, gene expression, proliferation, and differentiation, playing an indispensable central role in the construction of the blood-brain barrier, maintenance of tissue homeostasis, and pathological processes including inflammation and tumor metastasis.
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Picture
Western Blot
WB result of Occludin Recombinant Rabbit mAb
Primary antibody: Occludin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: bEnd.3 whole cell lysate 20 µg
Lane 2: mouse colon lysate 20 µg
Lane 3: mouse small intestine lysate 20 µg
Lane 4: mouse liver lysate 20 µg
Lane 5: mouse kidney lysate 20 µg
Lane 6: mouse brain lysate 20 µg
Lane 7: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 18-60 kDa
WB result of Occludin Recombinant Rabbit mAb
Primary antibody: Occludin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat colon lysate 20 µg
Lane 2: rat liver lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Lane 4: rat brain lysate 20 µg
Lane 5: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 18-60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Caco2 cells. Anti-Occludin antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
