WB result of Nucleostemin Recombinant Rabbit mAb
Primary antibody: Nucleostemin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 65 kDa
Nucleostemin Recombinant Rabbit mAb (S-1888-40)
Nucleostemin Recombinant Rabbit mAb (S-1888-40)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Nucleostemin |
| Synonyms | Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47 (NNP47); Nucleolar GTP-binding protein 3; E2IG3; NS; GNL3 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9BVP2 |
| Clone Number | S-1888-40 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, Raji, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Nucleostemin (NS), also known as guanine nucleotide-binding protein-like 3 (GNL3), is a nucleolar GTP-binding protein predominantly found in the nucleoli of stem cells, transformed cell lines, and various tumor cells. Initially identified in rat neural stem cells, NS is highly conserved across species and plays a crucial role in cell-cycle progression. It regulates cell proliferation by modulating ribosomal biogenesis and interacting with the p53-MDM2 feedback loop. Interestingly, both overexpression and depletion of NS can activate p53, suggesting its dual role in cellular homeostasis. Additionally, NS levels decrease as cells differentiate, making it a potential marker for stem cells and cancer cells. Its unique functions in ribosome assembly and cell-cycle regulation highlight its importance in both normal development and tumorigenesis.
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Western Blot
WB result of Nucleostemin Recombinant Rabbit mAb
Primary antibody: Nucleostemin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 70 kDa
WB result of Nucleostemin Recombinant Rabbit mAb
Primary antibody: Nucleostemin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 75 kDa
