WB result of NTRK1/TrkA Recombinant Rabbit mAb
Primary antibody: NTRK1/TrkA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 140 kDa
This blot was developed with high sensitivity substrate
NTRK1/TrkA Recombinant Rabbit mAb (S-2524-16)
NTRK1/TrkA Recombinant Rabbit mAb (S-2524-16)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NTRK1/TrkA |
| Synonyms | High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A (Trk-A); MTC; TRK; TRKA |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P04629 |
| Clone Number | S-2524-16 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | TF-1, K562, SH-SY5Y |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
Background
NTRK1/TrkA, encoded on chromosome 1q23.1, is a 140 kDa high-affinity receptor tyrosine kinase for nerve growth factor (NGF) that, upon ligand-induced dimerization and autophosphorylation at Tyr490, Tyr751 and Tyr785, activates the PI3K/Akt, Ras/MAPK and PLCγ pathways to orchestrate neuronal survival, differentiation, nociception and evasion of apoptosis, while its fusion proteins or mutations can drive oncogenesis and are targeted by TRK inhibitors.
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Western Blot
