WB result of NSUN2 Mouse mAb
Primary antibody: NSUN2 Mouse mAb at 1/5000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: Caco2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 105 kDa
NSUN2 Mouse mAb
NSUN2 Mouse mAb
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | NSUN2 |
| Synonyms | RNA cytosine C(5)-methyltransferase NSUN2; Myc-induced SUN domain-containing protein (Misu); NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; mRNA cytosine C(5)-methyltransferase; tRNA cytosine C(5)-methyltransferase (; ); tRNA methyltransferase 4 homolog (hTrm4); SAKI; TRM4; NSUN2 |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | Q08J23 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HEK-293, Caco2, HepG2, Jurkat |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu |
| IHC-P | 1:500 | Hu |
Background
NSUN2 (NOP2/Sun domain family member 2) is a crucial RNA methyltransferase enzyme responsible for catalyzing the 5-methylcytosine (m5C) modification on various RNA species, including tRNAs, mRNAs, and rRNAs. As a key epitranscriptomic regulator, it plays an essential role in post-transcriptional gene expression by influencing RNA stability, nuclear export, and translation efficiency. Furthermore, NSUN2 is deeply involved in fundamental biological processes such as cell proliferation, stem cell differentiation, and development, while its dysregulation is frequently associated with the initiation and progression of various cancers.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-NSUN2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-NSUN2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-NSUN2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
