NRAS enzyme titration
NRAS, Avi&His Tag Protein
NRAS, Avi&His Tag Protein
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$774 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | ALPS4, CMNS, N-ras, NCMS, NRAS1, NS6 |
| Accession | P01111 |
| Amino Acid Sequence |
M1-N172 MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLN |
| Expression System | E.coli |
| Molecular Weight | 23.6 kDa |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Stability & Storage | -80℃ |
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Bioactivity
The NRAS activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration
of BI-2852, NRAS protein, substrate and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
SDS-PAGE
