WB result of NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb
Primary antibody: NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 μg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: Y79 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 57 kDa
NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb (S-5962)
NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb (S-5962)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NR1D1 + NR1D2 / RVR |
| Synonyms | Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1 (EAR-1); EAR1; HREV; THRAL; Nuclear receptor subfamily 1 group D member 2; Orphan nuclear hormone receptor BD73; Rev-erb alpha-related receptor (RVR); Rev-erb-beta; V-erbA-related protein 1-related (EAR-1R) |
| Location | Cytoplasm, Nucleus, Synapse |
| Accession | P20393、 Q14995 |
| Clone Number | S-5962 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A549, HeLa, HepG2, Jurkat, 293T, Y79, NIH/3T3, RAW264.7, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
NR1D1 and NR1D2 are unique transcriptional repressors within the nuclear receptor family that serve as core components of the molecular circadian clock, intricately bridging circadian rhythm regulation with a wide range of physiological and pathological processes in the body. These two proteins lack a classical ligand-binding pocket and instead sense cellular metabolic states by binding heme as a cofactor, exerting transcriptional repression through their N-terminal DNA-binding domains that recognize specific response elements. In the negative feedback loop of the circadian clock, they directly repress the transcription of the core clock gene BMAL1, maintaining the stability of the approximately 24-hour circadian periodicity. Although the two proteins are highly synergistic in function, sharing over 50% of their DNA-binding sites and exhibiting overlapping and compensatory effects in transcriptional regulation, they also display significant isoform-specific characteristics: NR1D1 plays a particularly prominent role in regulating NF-κB-mediated inflammatory signaling pathways and lipid metabolism, while NR1D2 is indispensable for maintaining the expression of extracellular matrix components and for certain aspects of tissue development. This dual nature makes them potential therapeutic targets in various diseases—in the metabolic arena, they synergistically suppress lipogenesis and regulate hepatic glucose output; in the nervous system, double knockout of both proteins alleviates transcriptional repression of STAT3, thereby inducing astrocyte activation, yet interestingly, in Parkinson's disease models, this activation paradoxically enhances the clearance of α-synuclein and alleviates pathology; furthermore, they are deeply involved in the regulation of inflammatory responses, vascular smooth muscle cell remodeling, and metabolic reprogramming of tumor cells, highlighting the broad research prospects of REV-ERB proteins as a bridge from circadian biology to disease therapeutics.
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Western Blot
WB result of NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb
Primary antibody: NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 μg
Lane 2: Raw 264.7 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 57 kDa
WB result of NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb
Primary antibody: NR1D1 + NR1D2 / RVR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 57 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-NR1D1+NR1D2/RVR antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human uterus. Anti-NR1D1+NR1D2/RVR antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-NR1D1+NR1D2/RVR antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-NR1D1+NR1D2/RVR antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-NR1D1+NR1D2/RVR antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-NR1D1+NR1D2/RVR antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-NR1D1 + NR1D2 / RVR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
