NPM1 Rabbit Polyclonal Antibody

Catalog Number: S0B6891 Application: WB, IHC-P Reactivity: Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	NPM1
Synonyms	Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; Npm1
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cytoskeleton, Nucleus
Accession	Q61937
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms, Rt, Mk
Positive Sample	NIH/3T3, 3T3-L1, C2C12, PC-12, COS-7
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Ms, Rt, Mk
IHC-P	1:125-1:250	Ms, Rt

Background

Nucleophosmin (NPM1), also known as B23 or numatrin, is a highly abundant, multifunctional phosphoprotein primarily localized in the granular component of the nucleolus, where it plays pivotal roles in ribosome biogenesis, centrosome duplication, and the regulation of genomic stability. As a molecular chaperone with histone-binding activity, NPM1 facilitates the assembly and transport of ribosomal subunits from the nucleolus to the cytoplasm, thereby ensuring efficient protein synthesis, while also participating in the stress response by shuttling between the nucleus and cytoplasm to regulate the stability and activity of key tumor suppressors such as p53 and ARF. Structurally characterized by its ability to form pentameric rings and undergo liquid-liquid phase separation, NPM1 is crucial for maintaining nucleolar architecture; however, its dysfunction, particularly through cytoplasmic mislocalization caused by specific mutations like NPM1c+, is a defining driver event in acute myeloid leukemia (AML), highlighting its dual significance as both an essential housekeeping protein and a critical oncoprotein when deregulated.

Picture

Western Blot

WB result of NPM1 Rabbit pAb
Primary antibody: NPM1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 3T3-L1 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDa

WB result of NPM1 Rabbit pAb
Primary antibody: NPM1 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDa

WB result of NPM1 Rabbit pAb
Primary antibody: NPM1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 38 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-NPM1 antibody was used at 1/125 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.