WB result of NOX4 Recombinant Rabbit mAb
Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HEK-293 whole cell lysate 20 µg
Lane 2: unboiled BxPC-3 whole cell lysate 20 µg
Lane 3: unboiled HeLa whole cell lysate 20 µg
Lane 4: unboiled U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 62 kDa
NOX4 Recombinant Rabbit mAb (S-2246-45)
NOX4 Recombinant Rabbit mAb (S-2246-45)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NOX4 |
| Synonyms | NADPH oxidase 4; Kidney oxidase-1 (KOX-1); Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; RENOX |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane, Endoplasmic reticulum membrane |
| Accession | Q9NPH5 |
| Clone Number | S-2246-45 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, BxPC-3, HeLa, U-87, Neuro-2a, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
NOX4, a constitutively active member of the NADPH oxidase family, is widely expressed in human tissues (including kidney, heart, liver, and brain) and localizes primarily to the outer mitochondrial membrane, where it catalyzes the reduction of molecular oxygen to hydrogen peroxide (H₂O₂) without requiring cytosolic regulatory subunits; it associates only with p22phox and can be further activated by Poldip2. Functionally, NOX4-generated reactive oxygen species (ROS) regulate diverse cellular processes such as proliferation, differentiation, apoptosis, senescence, and mitochondrial dysfunction, and it contributes to pathologies including cancer (e.g., breast and kidney cancer), fibrosis, atherosclerosis, and stroke. Recent studies also reveal NOX4's presence in the nucleus of kidney cancer cells, where its expression predicts resistance to targeted therapies and poor survival .
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Western Blot
WB result of NOX4 Recombinant Rabbit mAb
Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 62 kDa
WB result of NOX4 Recombinant Rabbit mAb
Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 62 kDa
WB result of NOX4 Recombinant Rabbit mAb
Primary antibody: NOX4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 62 kDa
This blot was developed with high sensitivity substrate
