IHC shows positive staining in paraffin-embedded human spleen. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
NOX2/gp91phox Recombinant Rabbit mAb (S-2974-75)
NOX2/gp91phox Recombinant Rabbit mAb (S-2974-75)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NOX2/gp91phox |
| Synonyms | NADPH oxidase 2; CGD91-phox; Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta); Cytochrome b-245 heavy chain; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; CYBB |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P04839 |
| Clone Number | S-2974-75 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
NOX2/gp91phox, encoded by the CYBB gene on chromosome X, is a 570-amino-acid, ~91 kDa glycosylated flavohemoprotein that forms the catalytic core of the phagocyte NADPH oxidase complex; its N-terminal six-transmembrane α-helical domain anchors it to the plasma membrane, binds p22phox, and ligates two non-identical hemes that sequentially transfer electrons across the membrane, while its C-terminal cytoplasmic tail docks NADPH and FAD, enabling a four-step redox cascade that reduces extracellular O₂ to microbicidal superoxide (O₂⁻), thereby driving the respiratory burst essential for killing invading pathogens; genetic loss-of-function mutations in gp91phox cause chronic granulomatous disease, whereas its overactivation contributes to oxidative stress implicated in atherosclerosis, hepatic fibrosis, and other inflammatory disorders.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
