WB result of NOVA1 Recombinant Rabbit mAb
Primary antibody: NOVA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrate
NOVA1 Recombinant Rabbit mAb (S-4031-71)
NOVA1 Recombinant Rabbit mAb (S-4031-71)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NOVA1 |
| Synonyms | RNA-binding protein Nova-1; Neuro-oncological ventral antigen 1; Ventral neuron-specific protein 1; Nova1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9JKN6 |
| Clone Number | S-4031-71 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Ms, Rt |
| Positive Sample | mouse brain, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:250 | Ms, Rt |
| ICC | 1:500 | Rt |
| IF | 1:500 | Ms |
Background
NOVA1 (neuro-oncological ventral antigen 1) is a neuron-specific splicing factor characterized as an RNA-binding protein, belonging to a protein family that contains three KH domains, and is highly expressed primarily in the brain and nervous system. It specifically recognizes YCAY motifs (where Y represents a pyrimidine) on target RNAs and coordinates with other splicing factors to regulate the alternative splicing of numerous key neuronal genes, including those involved in synaptic transmission, axon guidance, and neuronal differentiation. NOVA1 plays a central role in nervous system development, synaptic plasticity maintenance, and neural circuit formation, and its dysfunction is closely associated with tumors of the central nervous system, such as paraneoplastic opsoclonus-myoclonus-ataxia syndrome. Gene knockout mouse experiments have shown that NOVA1 deficiency leads to abnormal neuronal migration, synaptic dysfunction, and severe behavioral abnormalities, including seizures and developmental delay. Furthermore, recent studies have revealed that NOVA1 influences addictive behaviors (such as the rewarding effects of morphine) and the risk of autism spectrum disorder by regulating the alternative splicing of specific genes, further highlighting its critical role in neuropathology.
Picture
Picture
Western Blot
WB result of NOVA1 Recombinant Rabbit mAb
Primary antibody: NOVA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NOVA1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-NOVA1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in PC-12 cells. Anti-NOVA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NOVA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
