WB result of Noggin Recombinant Rabbit mAb
Primary antibody: Noggin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
Noggin Recombinant Rabbit mAb (S-4030-12)
Noggin Recombinant Rabbit mAb (S-4030-12)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Noggin |
| Synonyms | NOG |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | Q13253 |
| Clone Number | S-4030-12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Rt, Mk |
| Positive Sample | PC-3, PANC-1, rat brain, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Rt, Mk |
Background
Noggin is a secreted glycoprotein that functions as a potent antagonist of bone morphogenetic proteins (BMPs), playing a pivotal role in embryonic development and tissue homeostasis by binding directly to BMPs with high affinity to prevent their interaction with cell surface receptors. Originally identified for its ability to induce neural tissue in Xenopus embryos, Noggin is essential for dorsal-ventral patterning, neural tube formation, somite segmentation, and joint morphogenesis, effectively acting as a crucial regulator that balances BMP signaling gradients during organogenesis. Beyond its developmental significance, Noggin continues to influence adult physiological processes, including neurogenesis and bone remodeling, and its dysregulation has been implicated in various pathological conditions such as heterotopic ossification, skeletal malformations, and certain cancers, making it a significant subject of study in both developmental biology and therapeutic research.
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Western Blot
WB result of Noggin Recombinant Rabbit mAb
Primary antibody: Noggin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
WB result of Noggin Recombinant Rabbit mAb
Primary antibody: Noggin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
