Noggin Protein, Human

Catalog Number: UA040560 Brand: UA BIOSCIENCE

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$120 USD

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Product Details

Product Specification

Species	Human
Synonyms	Noggin, SYM1, SYNS1, NOG
Accession	Q13253
Amino Acid Sequence	Gln28-Cys232
Expression System	CHO
Molecular Weight	26-35 kDa (Reducing)
Purity	>95% by SDS-PAGE.
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	No Tag
Physical Appearance	Lyophilized powder
Storage Buffer	PBS, 5% Trehalose, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. ·3 months, -20 to -80℃ under sterile conditions after reconstitution. ·1 week, 2 to 8℃ under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles.
Reference	Cell. 1996 Aug 23;86(4):599-606. Nature. 2002 Dec 12;420(6916):636-42.

Background

Human Noggin is a secreted homodimeric glycoprotein encoded by the NOG gene (17q22). Originally identified in Xenopus as the dorsalizing factor from Spemann’s organizer, it is a pivotal antagonist of bone morphogenetic proteins (BMPs). Noggin is essential for embryonic axis formation, neural induction, somite patterning, and joint morphogenesis; loss-of-function mutations in humans cause proximal symphalangism (SYM1) and multiple synostoses syndrome.

Noggin binds BMP-2, BMP-4, and BMP-7 with high affinity, physically blocking their interaction with type I and type II serine/threonine kinase receptors. This prevents receptor oligomerization and downstream SMAD1/5/8 phosphorylation, thereby inhibiting BMP-driven transcriptional programs. Its cystine-knot structure confers thermal stability and potent antagonistic activity, making recombinant human Noggin a standard reagent in stem-cell and developmental biology.

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Bioactivity

Measured by its ability to inhibit the activation of Human BMP (Luc) HEK293T Reporter cell by Human BMP-2 Protein. The EC₅₀ for this effect is 59 ng/ml.

Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected IC50 for this effect is 0.89-1.8 ng/ml of Noggin.

Noggin Protein, Human (Cat#UA040560) maintains lung cancer organoid growth well during multiple passages and long-term culture processes.

Noggin Protein, Human(Cat#UA040560) maintains colorectal cancer organoid growth well during multiple passages and long-term culture processes.

Immunofluorescence staining showed co‑expression of TTF1 (purple), Ki67 (green), and CK7 (red) in human lung cancer organoids, confirming their lung epithelial origin and proliferative activity.

Immunofluorescence staining showed co‑expression of CEA (purple), CDX2 (green), and SATB2 (red) in human colorectal cancer organoids, confirming their colorectal epithelial phenotype.