Measured by its ability to inhibit the activation of Human BMP (Luc) HEK293T Reporter cell by Human BMP-2 Protein. The EC50 for this effect is 59 ng/ml.
Noggin Protein, Human
Noggin Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Noggin, SYM1, SYNS1, NOG |
| Accession | Q13253 |
| Amino Acid Sequence | Gln28-Cys232 |
| Expression System | CHO |
| Molecular Weight | 26-35 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, 5% Trehalose, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Cell. 1996 Aug 23;86(4):599-606. |
Background
Human Noggin is a secreted homodimeric glycoprotein encoded by the NOG gene (17q22). Originally identified in Xenopus as the dorsalizing factor from Spemann’s organizer, it is a pivotal antagonist of bone morphogenetic proteins (BMPs). Noggin is essential for embryonic axis formation, neural induction, somite patterning, and joint morphogenesis; loss-of-function mutations in humans cause proximal symphalangism (SYM1) and multiple synostoses syndrome.
Noggin binds BMP-2, BMP-4, and BMP-7 with high affinity, physically blocking their interaction with type I and type II serine/threonine kinase receptors. This prevents receptor oligomerization and downstream SMAD1/5/8 phosphorylation, thereby inhibiting BMP-driven transcriptional programs. Its cystine-knot structure confers thermal stability and potent antagonistic activity, making recombinant human Noggin a standard reagent in stem-cell and developmental biology.
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Bioactivity
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected IC50 for this effect is 0.89-1.8 ng/ml of Noggin.
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SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
