WB result of NMT1 Recombinant Rabbit mAb
Primary antibody: NMT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 60 kDa
NMT1 Recombinant Rabbit mAb (S-2986-17)
NMT1 Recombinant Rabbit mAb (S-2986-17)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NMT1 |
| Synonyms | Glycylpeptide N-tetradecanoyltransferase 1; Myristoyl-CoA:protein N-myristoyltransferase 1 (HsNMT1; NMT 1; Type I N-myristoyltransferase); Peptide N-myristoyltransferase 1; Protein-lysine myristoyltransferase NMT1; NMT |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P30419 |
| Clone Number | S-2986-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | SK-OV-3, HeLa, NIH/3T3, mouse brain, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
NMT1 is a cytosolic monomeric enzyme that catalyzes the irreversible, co-translational transfer of myristic acid from myristoyl-CoA to the N-terminal glycine of nascent polypeptides, a modification essential for membrane targeting, signal transduction, and stability of oncogenic kinases (e.g., Src, FAK), and its overexpression in cancers promotes proliferation, suppresses autophagy, and supports metabolic reprogramming through specific regulation of AMPKβ myristoylation, making it a validated therapeutic target.
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Picture
Western Blot
WB result of NMT1 Recombinant Rabbit mAb
Primary antibody: NMT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 60 kDa
WB result of NMT1 Recombinant Rabbit mAb
Primary antibody: NMT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
