WB result of NLRP3 Recombinant Rabbit mAb
Primary antibody: NLRP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 10 µg/ml LPS for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 118 kDa
NLRP3 Recombinant Rabbit mAb (S-3914-9)
NLRP3 Recombinant Rabbit mAb (S-3914-9)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NLRP3 |
| Synonyms | NACHT, LRR and PYD domains-containing protein 3; Cold autoinflammatory syndrome 1 protein homolog; Cryopyrin; Mast cell maturation-associated-inducible protein 1; PYRIN-containing APAF1-like protein 1; Cias1; Mmig1; Nalp3; Pypaf1; Nlrp3 |
| Location | Secreted, Cytoplasm, Cytoskeleton, Nucleus, Mitochondrion, Endoplasmic reticulum |
| Accession | Q8R4B8 |
| Clone Number | S-3914-9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Positive Sample | RAW264.7 treated with 10 µg/ml LPS for 8 hours |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) is a ~118 kDa cytosolic pattern-recognition receptor that assembles into the macromolecular “NLRP3 inflammasome” when confronted by a structurally diverse set of microbial toxins, RNA viruses, extracellular ATP, crystalline substances or metabolic danger signals; upon activation NLRP3 nucleates the adaptor ASC and pro-caspase-1 filaments, driving caspase-1–mediated proteolytic maturation of interleukin-1β/18 and gasdermin D, which triggers pyroptotic cell death and amplifies systemic inflammation, thereby placing NLRP3 at the hub of innate immunity, autoinflammatory syndromes and chronic metabolic disorders such as gout, atherosclerosis, Alzheimer’s disease and type-2 diabetes.
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Picture
Western Blot
