WB result of NLRP3 Recombinant Rabbit mAb
Primary antibody: NLRP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: untreated THP-1 whole cell lysate 20 µg
Lane 3: THP-1 treated with 80nM TPA for 16 hours and 1ug/ml LPS for 1h whole cell lysate 20 µg
Weak expression: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 118 kDa
This blot was developed with high sensitivity substrate
NLRP3 Recombinant Rabbit mAb (S-1390-58)
NLRP3 Recombinant Rabbit mAb (S-1390-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NLRP3 |
| Synonyms | NACHT, LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1 (CLR1.1); Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1; C1orf7; CIAS1; NALP3; PYPAF1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q96P20 |
| Clone Number | S-1390-58 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | THP-1 |
| Predicted Reactivity | M. mulatta |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
M13 bacteriophage is a filamentous virus that infects bacteria, particularly Escherichia coli (E. coli) strains showing the F-pilus. It is approximately 1 µm in length and 6 nm in width, consisting of a circular single-stranded DNA (ssDNA) molecule surrounded by a protein capsid. M13 has been extensively studied for its biological role and replication mechanism. Due to advancements in molecular biology techniques, M13 has become an intriguing nanotool for various applications. One of its notable capabilities is 'phage display,' which allows M13 to display fused peptides and proteins on its minor and major coat proteins. This has paved the way for studying protein-protein and peptide-protein interactions, developing molecular probes for cell imaging, and detecting microorganisms.
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Western Blot
