WB result of NINJ1 Recombinant Rabbit mAb
Primary antibody: NINJ1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
NINJ1 Recombinant Rabbit mAb (S-3109-56)
NINJ1 Recombinant Rabbit mAb (S-3109-56)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NINJ1 |
| Synonyms | Ninjurin-1; hNINJ1; Nerve injury-induced protein 1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q92982 |
| Clone Number | S-3109-56 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Mk |
| Positive Sample | HepG2, A549, HeLa, Jurkat, K562, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Mk |
| IHC-P | 1:1000 | Hu |
Background
NINJ1, a 16 kDa evolutionary-conserved double-transmembrane protein first discovered as a nerve-injury-induced adhesion molecule, has emerged as the key executioner of plasma-membrane rupture (PMR) downstream of all major lytic cell-death pathways (pyroptosis, apoptosis, necroptosis, ferroptosis, PANoptosis, cuproptosis and parthanatos); upon death stimuli it oligomerizes into amphipathic filaments or “cookie-cutter” rings that breach the lipid bilayer, releasing damage-associated molecular patterns and propagating inflammation, while also regulating leukocyte trafficking, TLR4 signaling and blood–brain-barrier integrity, making it a promising therapeutic target in ischemia, sepsis, neuroinflammation and cancer.
Picture
Picture
Western Blot
WB result of NINJ1 Recombinant Rabbit mAb
Primary antibody: NINJ1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
