WB result of Niemann Pick C1 Recombinant Rabbit mAb
Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled U-87 MG whole cell lysate 20 µg
Lane 2: unboiled HT-1080 whole cell lysate 20 µg
Lane 3: unboiled SK-BR-3 whole cell lysate 20 µg
Lane 4: unboiled PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 142 kDa
Observed MW: 150-250 kDa
Niemann Pick C1 Recombinant Rabbit mAb (S-5243)
Niemann Pick C1 Recombinant Rabbit mAb (S-5243)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Niemann Pick C1 |
| Synonyms | NPC intracellular cholesterol transporter 1; Niemann-Pick C1 protein; NPC1 |
| Location | Lysosome, Endosome |
| Accession | O15118 |
| Clone Number | S-5243 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | U-87 MG, HT-1080, SK-BR-3, PC-3, 3T3-L1, Neuro-2a, rat liver, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Niemann–Pick C1 (NPC1) is a large (~142 kDa) polytopic integral membrane glycoprotein that resides predominantly in the limiting membranes of late endosomes and lysosomes, where it functions as a sterol-sensing shuttle: its N-terminal “cholesterol-binding pocket” captures cholesterol (and other hydrophobic metabolites) from the luminal leaflet, a ring of 13 trans-membrane helices forms a hydrophobic conduit that is gated by cytosolic loop 6, and the C-terminal “NPC2-interacting” domain receives cholesterol from the soluble lysosomal protein NPC2, thereby enabling net export of cholesterol to the cytoplasmic leaflet for redistribution to other organelles; pathogenic mutations in NPC1 block this lipid efflux, causing the fatal neurovisceral disorder Niemann–Pick disease type C1 characterized by lysosomal lipid accumulation, neurodegeneration and premature death.
Picture
Picture
Western Blot
WB result of Niemann Pick C1 Recombinant Rabbit mAb
Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled 3T3-L1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 142 kDa
Observed MW: 160-230 kDa
WB result of Niemann Pick C1 Recombinant Rabbit mAb
Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 142 kDa
Observed MW: 160-230 kDa
WB result of Niemann Pick C1 Recombinant Rabbit mAb
Primary antibody: Niemann Pick C1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 142 kDa
Observed MW: 150-230 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti- Niemann Pick C1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-Niemann Pick C1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Neuro-2a cells. Anti-Niemann Pick C1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
