WB result of NF-κB2 p100 Recombinant Rabbit mAb
Primary antibody: NF-κB2 p100 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HDLM-2 whole cell lysate 20 µg
Lane 3: Karpas-299 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 97 kDa
Observed MW: 110 kDa
NF-κB2 p100 Recombinant Rabbit mAb (S-2502-62)
NF-κB2 p100 Recombinant Rabbit mAb (S-2502-62)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NF-κB2 p100 |
| Synonyms | Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10 (Lyt10); LYT10; NFKB2 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q00653 |
| Clone Number | S-2502-62 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Mk |
| Positive Sample | HeLa, HDLM-2, Karpas-299, MCF7, THP-1, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:50 | Hu |
Background
NF-κB2 p100 is a 100 kDa cytoplasmic precursor protein encoded by the nfkb2 gene that functions both as an IκB-like inhibitor of NF-κB—sequestering RelB and other subunits in the cytoplasm—and as the obligate source of the mature NF-κB2 p52 subunit generated via stimulus-induced, phosphorylation- and ubiquitin-dependent partial proteasomal processing triggered by the alternative NF-κB pathway kinases NIK and IKKα, thereby enabling p52-RelB dimers to translocate to the nucleus and drive transcriptional programs essential for lymphoid organogenesis, B-cell maturation and adaptive immunity.
Picture
Picture
Western Blot
WB result of NF-κB2 p100 Recombinant Rabbit mAb
Primary antibody: NF-κB2 p100 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 97 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti- NF-κB2 p100 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- NF-κB2 p100 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- NF-κB2 p100 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
