WB result of NeuroD2 Recombinant Rabbit mAb
Primary antibody: NeuroD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 48 kDa
This blot was developed with high sensitivity substrate
NeuroD2 Recombinant Rabbit mAb (S-3092-64)
NeuroD2 Recombinant Rabbit mAb (S-3092-64)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NeuroD2 |
| Synonyms | Neurogenic differentiation factor 2; Class A basic helix-loop-helix protein 1 (bHLHa1); NeuroD-related factor (NDRF); BHLHA1; NDRF; NEUROD2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q15784 |
| Clone Number | S-3092-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse cerebellum, mouse brain, rat cerebellum, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms |
| IF | 1:500 | Ms |
Background
NeuroD2 is a neuron-specific basic helix-loop-helix transcription factor that drives cortical projection neuron differentiation, radial migration and layer-specific identity by directly activating genes like Cdk5r1, Lrp8, Fezf2 and Satb2, while it fine-tunes store-operated Ca²⁺ entry by repressing STIM1, and its deletion disrupts thalamocortical barrel map formation, reduces excitability and produces autism-like motor and seizure phenotypes.
Picture
Picture
Western Blot
WB result of NeuroD2 Recombinant Rabbit mAb
Primary antibody: NeuroD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Lane 3: rat brain lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 48 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human hippocampus. Anti-NeuroD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse hippocampus. Anti-NeuroD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NeuroD2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-NeuroD2 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
