WB result of NeuroD1 Recombinant Rabbit mAb
Primary antibody: NeuroD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Y79 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
NeuroD1 Recombinant Rabbit mAb (SDT-3843-59)
NeuroD1 Recombinant Rabbit mAb (SDT-3843-59)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NeuroD1 |
| Synonyms | Neurogenic differentiation factor 1; NeuroD; NeuroD1Alternative nameClass A basic helix-loop-helix protein 3 (bHLHa3); BHLHA3; NEUROD1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13562 |
| Clone Number | SDT-3843-59 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Y79, β-TC-6, mouse cerebellum, rat cerebellum |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
NeuroD1 (Neurogenic Differentiation 1) is a basic helix-loop-helix (bHLH) transcription factor that serves as a master regulator in the development of the nervous system and pancreas, primarily functioning to commit progenitor cells to a neuronal fate by activating downstream neurogenic genes while simultaneously suppressing glial differentiation pathways. Expressed transiently during embryogenesis in specific regions of the central and peripheral nervous systems, NeuroD1 binds to E-box DNA sequences as a heterodimer with E-proteins to drive the expression of targets essential for neuronal maturation, migration, and survival, making it critical for the formation of structures such as the hippocampus, cerebellum, and retina; beyond its developmental role, it is also pivotal in pancreatic beta-cell differentiation and has been identified as a potent reprogramming factor capable of directly converting non-neuronal cells, including astrocytes and fibroblasts, into functional neurons, thereby holding significant therapeutic potential for treating neurodegenerative diseases and repairing neural injury through endogenous regeneration or cell replacement strategies.
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Western Blot
WB result of NeuroD1 Recombinant Rabbit mAb
Primary antibody: NeuroD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: β-TC-6 whole cell lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
WB result of NeuroD1 Recombinant Rabbit mAb
Primary antibody: NeuroD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse retina. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
