Nestin Recombinant Rabbit mAb (S-5471)

Catalog Number: S0B60408 Application: WB, IHC-P, IF Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Nestin
Synonyms	Nes
Location	Cytoplasm, Cytoskeleton
Accession	Q6P5H2
Clone Number	S-5471
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, IF
Reactivity	Ms, Rt
Positive Sample	E14 mouse embryo brain
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms
IHC-P	1:1000	Ms, Rt
IF	1:500	Ms

Background

Nestin is a cytoskeletal component belonging to class VI intermediate filaments, initially identified as a marker of neuroepithelial stem cells and neural progenitor cells. It is structurally and expressively unique, featuring a short N-terminus and an extremely long C-terminal domain. Its expression is developmentally regulated: it is highly expressed in proliferating, undifferentiated progenitor cells and rapidly downregulated upon terminal differentiation, where it is largely replaced by other intermediate filament proteins (such as neurofilaments). Beyond the nervous system, Nestin is broadly expressed in local stem/progenitor cells in various tissues including skeletal muscle, heart, pancreas, liver, and hair follicles. As a cytoskeletal protein, Nestin typically co-polymerizes with other intermediate filament proteins (such as vimentin) to form heteropolymeric intermediate filament networks, participating in maintaining cell morphology, regulating cell migration, proliferation, and differentiation. Due to its aberrant high expression in various tumors (such as glioblastoma, melanoma, pancreatic cancer, and prostate cancer), which is closely associated with tumor malignancy, invasiveness, and poor prognosis, Nestin has been widely used as a reliable marker and a potential therapeutic target for cancer stem cells in solid tumors including gliomas.

Picture

Western Blot

WB result of Nestin Recombinant Rabbit mAb
Primary antibody: Nestin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: E14 mouse embryo brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 207 kDa
Observed MW: 300-350 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse E14 embryo brain. Anti-Nestin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Nestin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-Nestin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded mouse kidney. Anti-Nestin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.