WB result of Nephrin Rabbit mAb
Primary antibody: Nephrin Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 200 kDa
(This blot was developed with high sensitivity substrate)
Nephrin Recombinant Rabbit mAb (S-543-63)
Nephrin Recombinant Rabbit mAb (S-543-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Renal glomerulus-specific cell adhesion receptor |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9QZS7 |
| Clone Number | S-543-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:500 |
Background
Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. The renal filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane, and the podocytes of epithelial cells.
Picture
Picture
Western Blot
WB result of Nephrin Rabbit mAb
Primary antibody: Nephrin Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 200 kDa
Exposure time: 180 s (This blot was developed with high sensitivity substrate)Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Nephrin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Nephrin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
