NDUFB8 Recombinant Rabbit mAb (S-4770-29)

Catalog Number: S0B60407 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	NDUFB8
Synonyms	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; Complex I-ASHI (CI-ASHI); NADH-ubiquinone oxidoreductase ASHI subunit
Immunogen	Recombinant Protein
Location	Mitochondrion
Accession	O95169
Clone Number	S-4770-29
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HCT116, HeLa, A549, 293T, SW480, HepG2, mouse brain, mouse heart, mouse kidney, rat brain, rat heart, rat kidney
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:100-1:500	Hu, Ms, Rt

Background

NDUFB8, namely NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, is a critical accessory subunit of mitochondrial respiratory chain Complex I (NADH:ubiquinone oxidoreductase). Although it does not directly participate in catalysis, it is essential for the stable assembly and normal function of Complex I, with its specific role being to stabilize the structural module where ubiquinone reduction occurs and to support the proton translocation pathway. This protein is located in the inner mitochondrial membrane. Biallelic loss-of-function mutations in its encoding gene, NDUFB8, lead to isolated Complex I deficiency, resulting in an autosomal recessive primary mitochondrial disease (namely nuclear-type mitochondrial Complex I deficiency type 32). Patients typically present with infantile-onset encephalomyopathy resembling Leigh syndrome, characterized by developmental delay, seizures, hypotonia, and lactic acidosis. In addition, abnormal expression or functional alterations of NDUFB8 are also associated with neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, and may serve as potential biomarkers for these conditions.

Picture

Western Blot

WB result of NDUFB8 Recombinant Rabbit mAb
Primary antibody: NDUFB8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: SW480 whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 20 kDa

WB result of NDUFB8 Recombinant Rabbit mAb
Primary antibody: NDUFB8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 20 kDa

WB result of NDUFB8 Recombinant Rabbit mAb
Primary antibody: NDUFB8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat heart lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 20 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NDUFB8 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-NDUFB8 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-NDUFB8 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-NDUFB8 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-NDUFB8 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.