NDUFA9 Recombinant Mouse mAb (S-5382)

Catalog Number: S0B60187 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Mouse
Antigen	NDUFA9
Synonyms	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; Complex I-39kD (CI-39kD); NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUFS2L
Location	Mitochondrion
Accession	Q16795
Clone Number	S-5382
Antibody Type	Recombinant mAb
Isotype	IgG1,k
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	WI-38, mouse heart, rat heart
Predicted Reactivity	Bv
Purification	Protein G
Concentration	1.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:10000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

NDUFA9 is a mitochondrial protein encoded by the NDUFA9 gene, functioning as a 39-kDa non-catalytic accessory subunit of respiratory chain complex I. After being synthesized in the cytoplasm, this protein is transported to the mitochondrial matrix and is primarily localized to the Q module of complex I, where it plays a critical role in maintaining the structural stability and normal function of complex I. Although NDUFA9 is not directly involved in the catalytic electron transfer reactions, it is essential for the proper assembly of complex I and the stabilization of late assembly intermediates, and it may also participate in recruiting the substrate ubiquinone-10. At the pathological level, homozygous or compound heterozygous mutations in the NDUFA9 gene can lead to nuclear type 26 mitochondrial complex I deficiency (MC1DN26), a disorder inherited in an autosomal recessive manner with a broad clinical spectrum, ranging from neonatal-lethal Leigh syndrome to childhood-onset progressive neurodevelopmental disorders with prominent dystonia. Affected patients typically present with developmental delay, dystonia, seizures, and abnormal MRI signals in the basal ganglia, and the underlying pathogenesis lies in the failure of complex I assembly and a significant reduction in its activity caused by these mutations.

Picture

Western Blot

WB result of NDUFA9 Recombinant mouse mAb
Primary antibody: NDUFA9 Recombinant mouse mAb at 1/1000 dilution
Lane 1: WI-38 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35 kDa

WB result of NDUFA9 Recombinant mouse mAb
Primary antibody: NDUFA9 Recombinant mouse mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35 kDa

WB result of NDUFA9 Recombinant mouse mAb
Primary antibody: NDUFA9 Recombinant mouse mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 35 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-NDUFA9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.