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Napsin A Recombinant Rabbit mAb (SDT-2777-115)

Napsin A Recombinant Rabbit mAb (SDT-2777-115)

Catalog Number: S0B2442 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Napsin A
Synonyms Napsin-A; Aspartyl protease 4 (ASP4; Asp 4); Napsin-1; TA01/TA02; NAPSA; NAP1; NAPA
Immunogen Recombinant Protein
Location Secreted
Accession O96009
Clone Number SDT-2777-115
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample HCC78, HCC827
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:100 Hu
IHC-P 1:2000 Hu

Background

Napsin A is a pepsin-like aspartic proteinase predominantly expressed in type II pneumocytes and renal tubular cells; it is widely used as an immunohistochemical marker because its strong cytoplasmic staining is present in ~85 % of lung adenocarcinomas while being virtually absent in squamous cell carcinomas, making it valuable for distinguishing primary pulmonary adenocarcinoma from other tumor types.

Picture

Western Blot

WB result of Napsin A Recombinant Rabbit mAb
Primary antibody: Napsin A Recombinant Rabbit mAb at 1/100 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HCC78 whole cell lysate 20 µg
Lane 3: HCC827 whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 30-40 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human lung. Anti-Napsin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-Napsin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Napsin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Napsin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Napsin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.