WB result of Nanog Recombinant Rabbit mAb
Primary antibody: Nanog Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NCCIT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 38 kDa
Nanog Recombinant Rabbit mAb (S-1823-8)
Nanog Recombinant Rabbit mAb (S-1823-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Nanog |
| Synonyms | Homeobox protein NANOG; Homeobox transcription factor Nanog (hNanog) |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9H9S0 |
| Clone Number | S-1823-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Positive Sample | NCCIT |
| Predicted Reactivity | Cz, CyMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:1000 | Hu |
Background
Nanog protein is a key transcription factor crucial for maintaining pluripotency in mammalian early embryos and pluripotent stem cells. It contains a homeodomain and is involved in various cellular processes, including self-renewal and reprogramming. Nanog is essential for the acquisition of pluripotency and the reactivation of the X chromosome, playing a central role in the transcriptional network of pluripotency. It forms autoregulatory loops to maintain embryonic stem cell (ESC) identity and is indispensable for the transition to full pluripotency during reprogramming. Nanog's expression is complex and regulated at multiple levels, and it is associated with diseases such as teratocarcinoma and germ cell and embryonal cancer. Additionally, Nanog has been implicated in cancer progression, acting as a cancer stem cell marker and promoting the self-renewal and long-term proliferative potential of stem cells.
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Picture
Western Blot
IP
Nanog Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Nanog in 0.4 mg NCCIT whole cell lysate.
Western blot was performed on the immunoprecipitate using Nanog Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: NCCIT whole cell lysate 40 µg (Input)
Lane 2: Nanog Recombinant Rabbit mAb IP in NCCIT whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NCCIT whole cell lysate
Predicted MW: 35 kDa
Observed MW: 38 kDa
Exposure time: 180 s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human seminoma. Anti-Nanog antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Nanog antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
