Flow cytometric analysis of Mouse blood cells labelling CD3 antibody at 1/500 (0.1 μg) dilution/ (Red) compared with a Rat monoclonal IgG (Black) isotype control. Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
NA/LE Rat anti-mouse CD3 Recombinant mAb
NA/LE Rat anti-mouse CD3 Recombinant mAb
Price:
Regular price
$487.00 SGD
Regular price
Sale price
$487.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD3 |
| Synonyms | T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, Cd3z, Tcrz |
| Location | Cell membrane |
| Accession | P24161 |
| Clone Number | 17A2 |
| Antibody Type | Recombinant mAb |
| Isotype | Rat IgG2b, κ |
| Application | FCM, in vitro T cell stimulation/activation |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | Lot specific* (generally 0.5 to 5 mg/ml)* |
| Endotoxin | ＜1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| FCM | 1:500 |
Background
This antibody recognizes the same epitope as clone 17A2.
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. CD3 is associated with an heterodimer creating the T-cell receptor (TCR). The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines.
Picture
Picture
FC
Flow cytometric analysis of Mouse splenocytes labelling CD3 antibody at 1/500 (0.1 μg) dilution/ (Red) compared with a Rat monoclonal IgG (Black) isotype control. Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
