IF shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) (SDT-329-12)
N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) (SDT-329-12)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | N-Cadherin |
| Synonyms | Cadherin-2; CDw325; Neural cadherin (N-cadherin); CD325; CDHN; NCAD; CDH2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P19022 |
| Clone Number | SDT-329-12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Cy5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| IF | 1:500 | Hu, Ms, Rt |
Background
CD325, also known as N-cadherin or cadherin-2, is a 130–140 kDa type I transmembrane glycoprotein belonging to the cadherin superfamily. It is a calcium-dependent cell-cell adhesion molecule that plays a crucial role in maintaining tissue architecture and regulating morphogenetic movements during development. The protein consists of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. The extracellular domains mediate homophilic interactions between adjacent cells, while the cytoplasmic tail interacts with catenin proteins, which link the cadherin complex to the actin cytoskeleton. CD325 is expressed in various tissues, including neurons, cardiac muscle, and some cancer cells, and is involved in processes such as neural development, cardiac function, and cancer metastasis.
Picture
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Immunofluorescence
Negative control: IF shows negative staining in paraffin-embedded human chromophobe renal carcinoma. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cardiac muscle. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti-N-Cadherin Recombinant Rabbit mAb (Cy5 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
