IHC shows positive staining in paraffin-embedded human rhabdomyosarcoma. Anti-Myogenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Myogenin Recombinant Rabbit mAb (SDT-3351)
Myogenin Recombinant Rabbit mAb (SDT-3351)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Myogenin |
| Synonyms | Class C basic helix-loop-helix protein 3 (bHLHc3); Myogenic factor 4 (Myf-4); BHLHC3; MYF4; MYOG |
| Location | Nucleus |
| Accession | P15173 |
| Clone Number | SDT-3351 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:250 | Hu |
Background
Myogenin is a basic helix-loop-helix transcription factor encoded by the MYOG gene that serves as the master regulator of skeletal muscle differentiation, binding to E-box motifs in promoters of muscle-specific genes like desmin, myosin heavy chain, and the acetylcholine receptor to orchestrate terminal differentiation and fusion of myoblasts into multinucleated myotubes; its expression is induced by the upstream myogenic regulatory factors MyoD and Myf5, and its activity is modulated by interactions with E proteins, p300/CBP coactivators, and MEF2 family members, while in pathophysiology MYOG mutations or altered expression are linked to congenital myopathies and its ectopic expression in tumors is exploited as a sensitive immunohistochemical marker for rhabdomyosarcoma.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human neuroblastoma. Anti-Myogenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded Ewing’s sarcoma. Anti-Myogenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
