IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
MYH7 Recombinant Rabbit mAb (SDT-2936-44)
MYH7 Recombinant Rabbit mAb (SDT-2936-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MYH7 |
| Synonyms | Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform (MyHC-slow); Myosin heavy chain; cardiac muscle beta isoform (MyHC-beta); MYHCB |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P12883 |
| Clone Number | SDT-2936-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| IF | 1:1000-1:2000 | Hu, Ms, Rt |
Background
MYH7, the slow/cardiac β-myosin heavy chain encoded by the MYH7 gene on chromosome 14q12, is the dominant motor protein of the human ventricle and type I skeletal muscle fibers, forming the hexameric thick-filament complex (two MYH7, two ELC, two RLC) whose ATP-driven conformational cycles generate ≈2 pN of force at 5–10 nm steps; pathogenic missense or truncating variants disrupt this chemomechanical cycle and the accompanying α-helical coiled-coil packing, producing a dosage-sensitive spectrum of disease—hypertrophic and dilated cardiomyopathy, myosin storage myopathy, Laing distal myopathy—characterized by hypertrophic sarcomeres, replacement fibrosis, and progressive heart or skeletal muscle failure, while physiologically MYH7 expression is induced by thyroid hormone, exercise, and β-adrenergic signaling that up-regulate slow oxidative programs, making the protein a central therapeutic target for small-molecule myosin inhibitors (mavacamten) and gene-editing strategies aimed at normalizing contractile kinetics and Ca²⁺ sensitivity.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-MYH7 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat skeletal muscle. Anti-MYH7 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
