Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized IL-2, T7, Myc transfected 293T (Human embryonic kidney epithelial cell) labelling Myc tag (PE-Cy7® Conjugate) antibody at 1/200 (0.5 μg) dilution/ (right panel) compared with a Rabbit IgG Isotype Control / (left panel).
Product Details
Product Details
Product Specification
Host | Rabbit |
Immunogen | Synthetic Peptide |
Clone Number | S-114-13 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | ICFCM |
Reactivity | Species Independent |
Purification | Protein A |
Concentration | 1 mg/ml |
Conjugation | PE-Cy7 |
Physical Appearance | Liquid |
Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
ICFCM | 1:200 |
Background
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
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