WB result of Myc Tag Recombinant Mouse mAb
Primary antibody: Myc Tag Recombinant Mouse mAb at 1/50000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with myc tag fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 14 kDa
Myc tag Recombinant Mouse mAb (S-5300)
Myc tag Recombinant Mouse mAb (S-5300)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Myc tag |
| Clone Number | S-5300 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Species independent |
| ICC | 1:1000 | Species independent |
Background
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in H3.3 (G34W mutant)-myc tag transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- myc tag antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
