WB result of MX1 Rabbit pAb
Primary antibody: MX1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml hIFN-α1 for 16 hours whole cell lysate 20 µg
Lane 3: untreated A549 whole cell lysate 20 µg
Lane 4: A549 treated with 10 ng/ml hIFN-α1 for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 80 kDa
MX1 Rabbit Polyclonal Antibody
MX1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MX1 |
| Synonyms | Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78 (IFI-78K); Interferon-regulated resistance GTP-binding protein MxA; Myxoma resistance protein 1; Myxovirus resistance protein 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Endoplasmic reticulum |
| Accession | P20591 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
MX1 (Myxovirus resistance protein 1) is an interferon-inducible dynamin-like large GTPase that constitutes a frontline intracellular defense against a broad spectrum of RNA and DNA viruses by recognizing viral nucleocapsids, oligomerizing into helical assemblies driven by GTP binding and hydrolysis, and sequestering or mis-sorting these capsids away from replication sites, thereby blocking viral transcription and genome amplification; the 662-aa human protein (also termed MxA) contains an N-terminal GTPase domain connected via a bundle-signalling element to a C-terminal stalk that together mediate self-assembly, while a disordered L4 loop and a unique N-terminal segment dictate its predominantly cytoplasmic localization, determine its extensive antiviral breadth including influenza A, vesicular stomatitis, bunyaviruses and HBV, and explain why polymorphisms or truncations in MX1 can abolish this activity and increase host susceptibility to infection.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-MX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MX1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of HeLa cells treated with hIFN-α1(10ng/ml, 16h) (top panel) and untreated HeLa cells (below panel). Anti- MX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
