MUC17 Recombinant Rabbit mAb (SDT-3951-108)

Catalog Number: S0B2501 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 500μl

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Product Details

Product Specification

Host	Rabbit
Antigen	MUC17
Immunogen	Recombinant Protein
Location	Cell membrane
Accession	Q685J3
Clone Number	SDT-3951-108
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	AsPC-1
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:500	Hu

Background

Mucin-17 (MUC17) is a membrane-tethered mucin and a member of the mucin family. Mucin family members play crucial roles in protecting epithelial cells, providing lubrication, maintaining epithelial integrity, aiding in cell adhesion, and offering protection against pathogens, toxins, and foreign particles. MUC17's expression changes have been observed in various pathological conditions, especially in cancer and inflammatory bowel diseases. The biosynthesis of MUC17 occurs in the endoplasmic reticulum of goblet cells and may undergo N-glycosylation either before or after dimerization. In tumor tissues, the abnormal expression of MUC17 is correlated with tumor invasion, metastasis, and prognosis. In recent years, MUC17 has been recognized as an important biomarker for certain cancers and a promising therapeutic target. Particularly, the role of MUC17 in gastric cancer (GC) has attracted researchers' attention. According to one study, MUC17 is highly expressed in gastric cancer tissues and is associated with a favorable prognosis for patients.

Picture

Western Blot

WB result of MUC17 Recombinant Rabbit mAb
Primary antibody: MUC17 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K-562 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: AsPC-1 whole cell lysate 20 µg
Negative control: K-562 whole cell lysate; HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 452 kDa
Observed MW: 452 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human small intestine. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer (case 1). Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer (case 2). Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MUC17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.