mTOR Recombinant Rabbit mAb (S-6467)

Catalog Number: S0B60798 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	mTOR
Location	Cytoplasm, Cell membrane, Endoplasmic reticulum membrane, Mitochondrion outer membrane
Accession	P42345
Clone Number	S-6467
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, MCF7, A549, MEF, K562, Raji, Hep G2, C2C12, C6, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

mTOR (mechanistic target of rapamycin) is a serine/threonine protein kinase of 2,549 amino acids (molecular weight ~280 kDa) encoded in the p36.2 region of human chromosome 1, belonging to the phosphatidylinositol 3-kinase-related kinase (PIKK) family; its C-terminus contains a kinase domain and includes regulatory domains such as FAT, FRB, and FATC, while its N-terminus contains approximately 20 HEAT repeats that mediate protein–protein interactions. Within cells, mTOR exists in two structurally and functionally distinct multiprotein complexes: mTORC1 is composed of mTOR, Raptor, mLST8, PRAS40, and Deptor and is sensitive to acute rapamycin treatment; mTORC2 is composed of mTOR, Rictor, mSin1, mLST8, and Protor, among others, and is insensitive to rapamycin. The core function of mTORC1 is to serve as a sensor of nutrient and energy status, integrating signals such as amino acid, growth factor, and ATP levels, and promoting protein synthesis by phosphorylating its key downstream substrates S6K1 and 4E-BP1 while simultaneously inhibiting catabolic processes such as autophagy, thereby maintaining a balance between anabolism and catabolism to regulate cell growth. Abnormal activation of the mTOR signaling pathway is closely associated with the occurrence and development of various tumors, making it an important target for cancer therapy; rapamycin and its analogs allosterically inhibit mTORC1 activity by forming a complex with FKBP12 that binds to the FRB domain.

Picture

Western Blot

WB result of mTOR Recombinant Rabbit mAb
Primary antibody: mTOR Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: HeLa whole cell lysate 20 μg
Lane 2: MCF7 whole cell lysate 20 μg
Lane 3: A549 whole cell lysate 20 μg
Lane 4: MEF whole cell lysate 20 μg
Lane 5: K-562 whole cell lysate 20 μg
Lane 6: Raji whole cell lysate 20 μg
Lane 7: Hep G2 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 289 kDa
Observed MW: 270 kDa

WB result of mTOR Recombinant Rabbit mAb
Primary antibody: mTOR Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: C2C12 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 289 kDa
Observed MW: 270 kDa

WB result of mTOR Recombinant Rabbit mAb
Primary antibody: mTOR Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: C6 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 289 kDa
Observed MW: 270 kDa

WB result of mTOR Recombinant Rabbit mAb
Primary antibody: mTOR Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: COS-7 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 289 kDa
Observed MW: 270 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brain. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-mTOR antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.