Mouse Inflammation 10-Plex Kit (Flow Cytometry Multiplex Bead Assay)
Mouse Inflammation 10-Plex Kit (Flow Cytometry Multiplex Bead Assay)
Product Details
Product Details
Product Specification
| Antigen | IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10、IL-12p70、TNF-α、IFN-γ、CXCL1/KC |
| Reactivity | Mouse |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: ＜5%； Inter-assay: ＜15% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | ＜3.0 pg/mL |
| Recovery | 70% - 130% |
| Assay time | 70 minutes |
| Species reactivity | Mouse |
Background
The Cytometric Bead Array (CBA) technology enables the capture of soluble analytes or analyte panels using beads with known sizes and fluorescence intensities, thereby allowing the detection of analytes via flow cytometry.
Each capture bead in the CBA kit is conjugated with a specific antibody. The detection reagent provided with the kit is Streptavidin-Phycoerythrin, and the intensity of the fluorescent signal generated by this reagent is proportional to the amount of bound analyte.
When the capture beads and detection reagent are incubated together with the test sample containing target analytes, sandwich complexes are formed. Detection of these complexes by flow cytometry allows for the identification of particles exhibiting the fluorescence characteristics of both the beads and the detection reagent.
