Mouse pulmonary cells were stained using the Mouse TE/TN/TCM/TEM Panel Kit consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC, anti-mouse CD4 PE, anti-mouse CD8 APC, anti-mouse CD44 PE-Cy7, and anti-mouse CD62L Pacific Blue conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs SSC-A dot plot show the T cells with the CD45+ gate applied.
4. Create a CD4 PerCP-Cy5.5-A vs CD8 APC-A dot plot with the CD3+ gate applied.
5. Create a CD62L Pacific Blue-A vs CD44 APC-Cy7-A dot plot and apply the CD8-CD4+ gate. Create gates to encompass CD62L+ CD44- cells (Naive T cells, Tn), CD62L+ CD44+ cells (Central memory T cells, Tcm), CD62L- CD44+ cells (Effector memory T cells, Tem), CD62L- CD44- cells (Teff).
Mouse TE/TN/TCM/TEM Panel Kit
Mouse TE/TN/TCM/TEM Panel Kit
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Ms |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This six‑color panel is designed for characterizing T‑cell subpopulations, including naïve T cells, central memory T cells, effector memory T cells, and short‑lived effector T cells in mouse tissue cells. CD45 was included as a general leukocyte marker to identify all hematopoietic cells. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. Finally, the expression pattern of CD44 and CD62L staining on T cells can distinguish distinct memory/effector subsets: naïve T cells (Tn), central memory T cells (Tcm), effector memory T cells (Tem), and effector T cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
Picture
Picture
FC
Mouse splenocytes were stained using the Mouse TE/TN/TCM/TEM Panel Kit consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC, anti-mouse CD4 PE, anti-mouse CD8 APC, anti-mouse CD44 PE-Cy7, and anti-mouse CD62L Pacific Blue conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the lymphocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs SSC-A dot plot show the T cells with the CD45+ gate applied.
4. Create a CD4 PerCP-Cy5.5-A vs CD8 APC-A dot plot with the CD3+ gate applied.
5. Create a CD62L Pacific Blue-A vs CD44 APC-Cy7-A dot plot and apply the CD8-CD4+ gate. Create gates to encompass CD62L+ CD44- cells (Naive T cells, Tn), CD62L+ CD44+ cells (Central memory T cells, Tcm), CD62L- CD44+ cells (Effector memory T cells, Tem), CD62L- CD44- cells (Teff).
Mouse intestinal were stained using the Mouse TE/TN/TCM/TEM Panel Kit consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC, anti-mouse CD4 PE, anti-mouse CD8 APC, anti-mouse CD44 PE-Cy7, and anti-mouse CD62L Pacific Blue conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs SSC-A dot plot show the T cells with the CD45+ gate applied.
4. Create a CD4 PerCP-Cy5.5-A vs CD8 APC-A dot plot with the CD3+ gate applied.
5. Create a CD62L Pacific Blue-A vs CD44 APC-Cy7-A dot plot and apply the CD8-CD4+ gate. Create gates to encompass CD62L+ CD44- cells (Naive T cells, Tn), CD62L+ CD44+ cells (Central memory T cells, Tcm), CD62L- CD44+ cells (Effector memory T cells, Tem), CD62L- CD44- cells (Teff).
DLS
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