Standard curve
Example of Mouse CCL17 standard curve in Assay Diluent #12Y.
Mouse & Rat CCL17 (Serum & Plasma) OneStep ELISA Kit
Mouse & Rat CCL17 (Serum & Plasma) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | CCL17 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.9%； Inter-assay: 6.6% |
| Sample type | Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 16.023 pg/mL |
| Range | 23.44 pg/mL – 1500 pg/mL |
| Recovery | Serum: 88% Plasma: 91% |
| Assay time | 60 minutes |
| Species reactivity | Ms; Rt |
Background
Mouse CCL17 (TARC) is a CC chemokine that acts as the primary ligand for CCR4, specifically recruiting Th2 cells and regulatory T cells (Tregs). Produced by dendritic cells and macrophages under inflammatory stimulation, it serves as a key mediator of type 2 immunity. Its elevated expression drives allergic pathologies such as asthma and atopic dermatitis by promoting leukocyte infiltration and Th2-mediated inflammation, making it both a biomarker and a promising therapeutic target in allergic disease research.
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ELISA
Linearity
The concentrations of Mouse CCL17 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 145.98 pg/mL in Mouse serum.
#2 sample is undiluted samples are as follows: The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 311.05 pg/mL in Rat serum.
To assess the linearity of the assay, The sample was spiked with high concentrations of Mouse CCL17 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, The sample was spiked with high concentrations of Mouse CCL17 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
