Mouse NK Cell Panel Kit (CD3/CD49b/NK1.1/CD335)

This four‑color panel is designed for detecting natural killer (NK) cells in mouse tissue cells. CD3 was included as a T‑cell marker to exclude T lymphocytes, allowing the identification of NK cells within the CD3‑negative population. CD49b and NK1.1 are markers often used to detect NK cells; it is important to note that NK1.1 is also expressed on NKT cells, but since the analysis is performed on the CD3‑negative gate, NKT cells are excluded. Additionally, CD335 (NKp46) is included as another NK‑cell marker, which can be used together with NK1.1 to further confirm the NK cell identity. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.