Naïve CD4+T cells were enriched from C57BL/6 mouse splenocytes using the Naïve CD4+ T Cell Isolation Kit, and a Starter EasyIso Separator. Cells were stained with anti-CD4 Pacific Blue, anti-CD44-FITC, anti-CD62L Alexa Fluor® 647 and gated on Live+. The naïve CD8+ T cell content (CD8+CD44-CD62L+) of the isolated fraction.
Product Details
Product Details
Product Specification
| Stability & Storage |
Store protected from light at +2 to +8 ℃. Do not
freeze.
|
Background
The Column-Free Mouse Naïve CD4+ T Cell
Isolation Kit enables the rapid purification of untouched Naïve CD4+ T cells
via immunomagnetic separation. This approach depletes non-target cells without
labeling or stimulating the desired Naïve CD4+ T cells, preserving native
phenotype and functional integrity for downstream cell culture and functional
assays.
Components
1. Mouse Naïve CD4+ T Cell Antibody Cocktail: biotin-conjugated monoclonal anti-mouse antibodies against antigens not expressed by Mouse Naïve CD4+ T cells, supplied in PBS containing 0.1% BSA;
2. Streptavidin Beads: NanoBeads conjugated to streptavidin, supplied in PBS containing BSA and Poloxamer 188.
Picture
Picture
Validation Data

