Schematic diagram of Mouse Myeloid Panel Kit (CD11b/Ly6C/CD11c/I-A/I-E)
Mouse Myeloid Panel Kit (CD11b/Ly6C/CD11c/I-A/I-E)
Mouse Myeloid Panel Kit (CD11b/Ly6C/CD11c/I-A/I-E)
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$250 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Ms |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This four‑color panel is designed for detecting myeloid cell subsets, including monocytes, resident macrophages, granulocytes, and conventional dendritic cells in mouse tissue cells. CD11b was included as a general myeloid cell marker. Ly‑6C was included to distinguish monocytes from resident macrophages and granulocytes. Finally, CD11c and I-A/I-E (MHC class II) can identify conventional dendritic cells. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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Picture
FC
Mouse bone marrow were stained using the Mouse Myeloid Panel Kit consisting of anti-mouse CD11b FITC, anti-mouse Ly-6C PE, anti-mouse CD11c APC and anti-mouse I-A/I-E PerCP-Cy5.5 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD11b FITC-A vs SSC-A dot plot show the myeloid cells with the CD45+ gate applied.
4. Create a CD11b FITC-A vs Ly-6C PE-A dot plot with the CD45+ gate applied. Create gates to encompass CD11b+Ly-6C+ cells (monocytes), CD11b+Ly-6C- cells (Resident macrophages and granulocytes)
5. Create a CD11c APC-A vs I-A/I-E PerCP-Cy5.5-A dot plot with the CD11b+ gate applied. Create gates to encompass CD11c+I-A/I-E+ cells (conventional dendritic cell, cDC)
Mouse hepatocytes were stained using the Mouse Myeloid Panel Kit consisting of anti-mouse CD11b FITC, anti-mouse Ly-6C PE, anti-mouse CD11c APC and anti-mouse I-A/I-E PerCP-Cy5.5 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b FITC-A vs SSC-A dot plot show the myeloid cells with the CD45+ gate applied.
4. Create a CD11b FITC-A vs Ly-6C PE-A dot plot with the CD45+ gate applied. Create gates to encompass CD11b+Ly-6C+ cells (monocytes), CD11b+Ly-6C- cells (Resident macrophages and granulocytes)
5. Create a CD11c APC-A vs I-A/I-E PerCP-Cy5.5-A dot plot with the CD11b+ gate applied. Create gates to encompass CD11c+I-A/I-E+ cells (conventional dendritic cell, cDC)
Mouse splenocytes were stained using the Mouse Myeloid Panel Kit consisting of anti-mouse CD11b FITC, anti-mouse Ly-6C PE, anti-mouse CD11c APC and anti-mouse I-A/I-E PerCP-Cy5.5 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the lymphocyte population.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD11b FITC-A vs SSC-A dot plot show the myeloid cells with the CD45+ gate applied.
4. Create a CD11b FITC-A vs Ly-6C PE-A dot plot with the CD45+ gate applied. Create gates to encompass CD11b+Ly-6C+ cells (monocytes), CD11b+Ly-6C- cells (Resident macrophages and granulocytes)
5. Create a CD11c APC-A vs I-A/I-E PerCP-Cy5.5-A dot plot with the CD11b+ gate applied. Create gates to encompass CD11c+I-A/I-E+ cells (conventional dendritic cell, cDC)
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