Schematic diagram of Mouse Immune Cell Subset Panel Kit.
Mouse Immune Cell Subset Panel Kit
Mouse Immune Cell Subset Panel Kit
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | Mouse splenocytes |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This seven‑color panel is designed for detecting immune cells in mouse tissue cells. CD45 was a general leukocyte marker to identify leukocytes. CD3 was a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. B cells were identified by CD19, which is a general B‑cell marker. NK cells were identified by CD49b. Finally, Ly6C was included as a marker for myeloid cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
Picture
Picture
FC
Mouse splenocytes were stained using the Mouse Immune Cell Subset Panel Kit consisting of anti-mouse CD45 Pacific Blue, anti-mouse CD3 Alexa Fluor® 488, anti-mouse CD4 PE-Cy7, anti-mouse CD8 PE, anti-mouse CD19 APC-Cy7, anti-mouse CD49b APC, and anti-mouse Ly6C PerCP-Cy5.5 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the lymphocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD3 Alexa Fluor® 488-A vs SSC-A dot plot with the CD45+ gate applied, and draw a gate around the CD3+ T cells.
4. Create a CD4 PE-Cy7-A vs CD8 PE-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
5. Create a CD3 Alexa Fluor® 488-A vs CD19 APC-Cy7-A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD19- cells (T cells), CD3-CD19+ cells (B cells).
6. Create a CD3 Alexa Fluor® 488-A vs CD49b APC-A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T-like cells), CD3-CD49b+ cells (NK cells).
7. Create a Ly6C PerCP-Cy5.5-A vs SSC-A dot plot and apply the CD45+ gate. Draw a gate around Ly6C^hi cells (enriched for inflammatory monocytes).
