Standard curve
Example of Mouse IL-6 standard curve in Assay Diluent #9.
Mouse IL-6 OneStep ELISA Kit
Mouse IL-6 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-6 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | 板内变异系数（Intra-assay）：4.1%； 板间变异系数（Inter-assay）：5.1% |
| Sample type | Cell culture supernatant, Serum, Plasm, Cell/Tissue Lysate |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.788 pg/ml |
| Range | 7.81 pg/mL - 500 pg/mL |
| Recovery | Cell culture supernatant: 102% Serum: 106% Plasm: 102% |
| Assay time | 60 minutes |
| Species reactivity | Mouse |
Background
Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.
Picture
Picture
ELISA
Linearity
The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: mouse heart cells culture for 5 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.17 ng/mL in mouse heart cells culture supernatant.
#2 sample is undiluted samples are as follows: RAW264.7 cells stimulated with 1ug/ml LPS for 48h (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.52 ng/mL in stimulated human RAW264.7 cells supernatant.
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