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Mouse IL-18 ELISA Kit

Mouse IL-18 ELISA Kit

Catalog Number: abs552215 Application: ELISA Reactivity: Mouse Conjugation:
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Regular price $381 USD
Regular price Sale price $381 USD
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Product Details

Product Specification

protein IL-18
Species Reactivity Mouse
Theory This kit uses double antibody sandwich ELISA method, and the experimental duration is 4 hours. The plates in the kit have been pre-coated with anti-IL-18 antibodies. Add the standard substance and moderately diluted sample to be tested into the corresponding wells respectively, and wash away the unbound components after incubation. A biotin-detection antibody is added which binds to IL-18 already bound to the coated antibody. After washing off the unbound components, HRP-streptavidin (SABC) was added. Then the unbound components were washed away, and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP), and turned yellow after the reaction stopper was added. The OD value was measured with a microplate reader at 450 nm wavelength. The concentration of IL-18 in the sample was calculated by drawing a standard curve. There is a direct proportion between the concentration of the target substance and the OD450 value.
Source Mouse
Synonym Mouse Interleukin 18 (IL-18/IL18) ELISA Kit
Description Sensitivity: 9.375 pg/ml
Detection wavelength: OD450
Specificity: Specifically binds to IL-18 without significant cross-reaction with other analogs
Detection Method Sandwich ELISA, Double Antibody
Test time: 4 hours (excluding equilibration and sample preparation time)
Composition
Serial number Name Specifications Save
1

Elisa Enzyme plate ( Detachable )
ELISA Microplate(Dismountable)

8 Hole ×12 Strip

Place the unused holes in a zippered aluminum foil bag and add desiccant and store sealed. Available at 2-8°C Save 1 Months; In -20°C Save 6 Months.

2

Lyophilized Standard
Lyophilized Standard

2 branch

Place the unused standard in the desiccant pack. Available at 2-8°C Save 1 Months; In -20°C Save 6 Months.

3

Concentrated biotin - antibody 100X
Biotin-labeled Antibody

1×120ul

2-8°C ( Protect from light )

4

concentrate HRP- Streptavidin 100X
HRP-Streptavidin Conjugate(SABC)

1×120ul

5

TMB Chromogenic substrate
TMB Substrate

1×10mL
6

Sample dilution
Sample Dilution Buffer

1×20mL 2-8℃
7

Antibody dilution
Antibody Dilution Buffer

1×10mL
8

SABC Diluent
SABC Dilution Buffer

1×10mL
9

Reaction stop solution
Stop Solution

1×10mL
10

Concentrated wash 25X
Wash Buffer 25X

1×30mL
11

Sealing film

3 Zhang  
General Notes

1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure.
2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture).
3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation.
4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tends to cause false positives and high backgrounds.
5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure.
6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation.
7. Do not reuse disposable tips to avoid cross-contamination.
8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature.
9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.

Instructions It is used for quantitative detection of IL-18 in serum, plasma, cell culture supernatant or other biological samples in vitro.
Storage Temp. 2-8 °C (when unopened), do not freeze, shelf life 12 months.
Test Range 15.625-1000pg/ml