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Mouse IL-33 High Sensitivity ELISA Kit

Mouse IL-33 High Sensitivity ELISA Kit

Catalog Number: abs552809 Application: ELISA Reactivity: Mouse Conjugation:
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Regular price $450 USD
Regular price Sale price $450 USD
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Product Details

Product Specification

Species Reactivity Mouse
Synonym C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL1F11, IL-1F11, IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin-33, NFHEV, NF-HEV, NF-HEVNFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2
Description

Detection principle:

This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Mouse IL-33 capture antibody Pre-coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process, present in the sample IL-33 Binding to solid phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody is added for incubation. After washing again, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-33 The concentration is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.

 

Test Type: Double antibody sandwich method

 

Form: Pre-coating 96 Orifice plate

 

Test Sample Type: Cell supernatant, serum, plasma

 

Sample Load: 100 μl

 

Kit Components: Pre-coating 96 Well plate, standard, biotin labeled IL-33 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.

 

Sensitivity: 0.26 pg/mL

 

Detection range: 7.81-500 pg/mL

 

Recovery Range: 80-102%

 

Save Method: 2-8℃

 

Standard graph:

 

 

Background:

Interleukin 33 (IL-33) Also known as NF-HEV And DVS 27 , yes IL-1 Family members, with a molecular weight of 30 kDa , by more than Various types of cell secretion, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells and Epithelial cells. IL-33 Inducible adjuvant T Cells, mast cells, eosinophils, and basophils secretion II Type cell Factor. IL-33 By interacting with the receptor ST2 And IL-1 Receptor accessory protein (IL1RAP) Binding, activation can promote polarization Th2 Cells secretion II Type cytokine ( Such as IL-5 And IL-13) Of NF-κB And MAP Extracellular molecules in the kinase signaling pathway to mediate their biological Learning function. Injection IL-33 After, it can be induced in vivo II Type cytokines, which are thought to induce severe pathological changes in mucosal organs.

Storage Temp. Stored at 2 ~ 8 ℃, the validity period is two years.