Standard curve
Example of IL-17A standard curve in Assay Diluent .
Mouse IL-17A Single Plex Kit (Flow Cytometry Bead Assay)
Mouse IL-17A Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-17A |
| Reactivity | Mouse |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 3 pg/mL |
| Range | 3.5 pg/mL-10000 pg/mL |
| Recovery | Plasma: 99% Serum: 125% Cell culture supernatant: 116% |
| Assay time | 70 minutes |
| Species reactivity | Mouse |
Background
Mouse IL-17A (also known as CTLA-8) is a pro-inflammatory cytokine and the signature effector molecule of Th17 cells, with additional sources including γδ T cells, iNKT cells, and type 3 innate lymphoid cells (ILC3). Functioning as a master regulator of mucosal immunity, IL-17A signals through the IL17RA–IL17RC heterodimeric receptor complex to activate NFκB and MAPK pathways, thereby inducing the production of proinflammatory cytokines (such as TNFα and IL6), chemokines (including CXCL1 and CXCL5), antimicrobial peptides, and matrix metalloproteinases. This signaling cascade orchestrates neutrophil recruitment and activation, positioning IL17A as a critical mediator of host defense against extracellular bacteria and fungi. However, dysregulated IL17A expression is pathologically linked to a broad spectrum of autoimmune and inflammatory diseases, including rheumatoid arthritis, psoriasis, and multiple sclerosis, and has also been implicated in airway hyperresponsiveness, inflammatory bone loss, and even neurodevelopmental disorders such as autism spectrum disorder. In the context of cancer, IL17A exhibits a controversial dual role, with reports indicating both tumorsuppressive and tumorpromoting activities depending on the microenvironment. Given its central role in bridging adaptive and innate immunity, quantitative detection of mouse IL17A serves as an invaluable tool for studying Th17 differentiation, autoimmunity, host defense mechanisms, and the inflammatory tumor microenvironment, making it a key biomarker for evaluating immune status and inflammatory pathology in diverse research models.
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Picture
CBA
Quantifiction of Mouse spleen cells
Stimulated sample: Mouse spleen cells were stimulated with 10 μg/mL ConA for 24h, and the average concentration of IL-17A in the supernatant was measured to be 803.6 pg/mL.
Unstimulated sample: Mouse spleen cells were cultured for 24h, and the average concentration of IL-17A in the supernatant was measured to be 0.74 pg/mL.
Linearity
After serially diluting the high-concentration mouse IL-17A combination protein using different sample matrices from different mouse strains, the concentration of mouse IL-17A was determined and interpolated through the target standard curve, and the effect of sample dilution was corrected.
Recovery
Within the detection range, the combined cytokine proteins were added to different matrices at high,medium and low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Recovery
Within the detection range, the combined cytokine proteins were added to different matrices at high,medium and low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Recovery
Within the detection range, the combined cytokine proteins were added to different matrices at high,medium and low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Protocol Diagram
