Mouse IL-13 Single Plex Kit (Flow Cytometry Bead Assay)

Catalog Number: S0Q3077 Reactivity: Mouse Conjugation: Brand: Starter

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$500 USD

Size: 1x96T

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Product Details

Product Specification

Antigen	IL-13
Reactivity	Mouse
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay:； Inter-assay:
Sample type	Serum; Plasma-EDTA; Plasma-Heparin; Plasma-Citrate; Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	1.02 pg/mL
Range	2.40 pg/mL-3700 pg/mL
Recovery	Serum、plasma and cell culture supernatant: 70%~130%
Assay time	70 minutes
Species reactivity	Mouse

Background

Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence identity to IL-4 and is capable of IL-4 independent signaling. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma.

Picture

CBA

Standard curve

Example of IL-13 standard curve in Assay Diluent .

Quantifiction of Mouse Splenocytes
Stimulated sample: Mouse Splenocytes were stimulated with 4.0 µg/mL ConA for 48h, and the average concentration of IL-13 in the supernatant was measured to be 9282.11 pg/mL.
Unstimulated sample: Mouse Splenocytes were cultured for 48h, and the average concentration of IL-13 in the supernatant was measured to be 50.09 pg/mL.

Linearity
The high-concentration mouse IL-13 protein was serially diluted using serum, plasma-EDTA, plasma-heparin, plasma-citrate(CD-1 (ICR)、C57BL/6、BALB/c) and cell culture supernatant matrices respectively. Then, the concentration of mouse IL-13 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.